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Otolaryngol Head Neck Surg. 2011 May;144(5):809-14. doi: 10.1177/0194599810396134.

B lymphocyte subsets in patients with rhinoscleroma.

Author information

1
Department of Otorhinolaryngology, Audiology, and Phoniatrics Giorgio Ferreri, Sapienza University of Rome, Rome, Italy.

Abstract

OBJECTIVE:

To identify the presence of B lymphocytes in the rhinoscleroma granulomas as a possible precursor of plasma cells, whose presence has always been described but whose role is still unclear.

STUDY DESIGN:

Case series with chart review.

SETTING:

Sapienza University of Rome.

METHODS:

The study was carried out on 6 patients (3 women, 3 men). The following parameters were examined for each patient: clinical manifestations, number of leukocytes, lymphocytes and lymphocyte subsets (CD3+, CD4+, CD8+, and CD19+) in blood samples, time from the onset of symptoms, biopsies, and expression of CD3, CD4, CD8, and CD20 antigens in tissue samples with immunohistochemical techniques.

RESULTS:

In this study, the values of CD3+, CD4+, and CD8+ T lymphocyte subsets in venous peripheral blood are in line with previously reported data, whereas CD19+ lymphocyte cells tended to show an ambiguous behavioral pattern. In tissue samples, approximately one-third of the T lymphocyte population showed a CD3+/CD8+ immunophenotype (cytotoxic/suppressor), and two-thirds of the T lymphocytes expressed a CD3+/CD4+ immunophenotype (helper/inducer). The authors also identified an unexpected large amount of CD20+ non-plasmacellular B cells in addition to the plasma cells usually detected in rhinoscleroma biopsies.

CONCLUSIONS:

This study demonstrates the presence of B lymphocytes in rhinoscleroma tissue. It is presumable that the mature B cells activated by antigenic stimulation of Klebsiella rhinoscleromatis are the precursors of the plasma cells typically found in the granuloma of rhinoscleroma.

PMID:
21493362
DOI:
10.1177/0194599810396134
[Indexed for MEDLINE]

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