The fluorescence theatre: a cost-effective device using theatre gels for fluorescent protein and dye screening

Can J Microbiol. 2011 Apr;57(4):339-42. doi: 10.1139/w11-010.

Abstract

Here we report a simple cost-effective device for screening colonies on plates for expression of the monomeric red fluorescent protein mRFP1 and the fluorescent dye Nile red. This device can be built from any simple light source, in our case a Quebec Colony Counter, and cost-effective theatre gels. The device can be assembled in as little as 20 min, and it produces excellent results when screening a large number of colonies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Fluorescence
  • Fluorescent Dyes / analysis*
  • Fluorescent Dyes / metabolism
  • Gels*
  • Luminescent Proteins / analysis
  • Luminescent Proteins / metabolism
  • Microbiological Techniques / economics
  • Microbiological Techniques / instrumentation
  • Microbiological Techniques / methods*
  • Red Fluorescent Protein

Substances

  • Fluorescent Dyes
  • Gels
  • Luminescent Proteins