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Eur J Cancer. 1990;26(10):1031-4.

Cells secreting tumour necrosis factor show enhanced metastasis in nude mice.

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Imperial Cancer Research Fund, London, U.K.


A tumour cell may acquire the ability to invade and metastasise via heritable changes in its genome and/or changes in the local environment. Chinese hamster ovary (CHO) cells transfected with the gene for human TNF (CHO/TNF cells) showed a greatly enhanced ability to invade peritoneal surfaces and metastasise in nude mice compared with cells transfected by the vector alone. In situ hybridisation with a riboprobe for human TNF showed that the CHO/TNF cells were actively transcribing this cytokine after in vivo injection. Neutralising antibodies to human TNF, both whole IgG and F(ab)2 fragments, abrogated the enhanced metastatic activity of the TNF-secreting cells. Thus transfection of a cytokine/growth-factor gene can confer a metastatic phenotype on the recipient cell.

[Indexed for MEDLINE]

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