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Mol Ecol Resour. 2011 May;11(3):562-6. doi: 10.1111/j.1755-0998.2011.02985.x. Epub 2011 Feb 6.

POLYSAT: an R package for polyploid microsatellite analysis.

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Department of Plant Sciences, Mail Stop 4, University of California, Davis, CA 95616, USA.


We present an R package to help remedy the lack of software for manipulating and analysing autopolyploid and allopolyploid microsatellite data. POLYSAT can handle genotype data of any ploidy, including populations of mixed ploidy, and assumes that allele copy number is always ambiguous in partial heterozygotes. It can import and export genotype data in eight different formats, calculate pairwise distances between individuals using a stepwise mutation and infinite alleles model, estimate ploidy based on allele counts and estimate allele frequencies and pairwise F(ST) values. This software is freely available through the Comprehensive R Archive Network ( and includes a thorough tutorial.

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