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Int J Radiat Biol. 2011 Aug;87(8):869-77. doi: 10.3109/09553002.2011.568575. Epub 2011 Apr 11.

An ear punch model for studying the effect of radiation on wound healing.

Author information

1
Departments of Medicine, Duke University Medical Center, Durham, North Carolina, USA.

Abstract

PURPOSE:

Radiation and wound combined injury represents a major clinical challenge because of the synergistic interactions that lead to higher morbidity and mortality than either insult would produce singly. The purpose of this study was to develop a mouse ear punch model to study the physiological mechanisms underlying radiation effects on healing wounds.

MATERIALS AND METHODS:

Surgical wounds were induced by a 2 mm surgical punch in the ear pinnae of MRL/MpJ mice. Photographs of the wounds were taken and the sizes of the ear punch wounds were quantified by image analysis. Local radiation to the ear was delivered by orthovoltage X-ray irradiator using a specially constructed jig that shields the other parts of body.

RESULTS:

Using this model, we demonstrated that local radiation to the wound area significantly delayed the healing of ear punch wounds in a dose-dependent fashion. The addition of sublethal whole body irradiation (7 Gy) further delayed the healing of ear punch wounds. These results were replicated in C57BL/6 mice; however, wound healing in MRL/MpJ mice was accelerated.

CONCLUSIONS:

These data indicate that the mouse ear punch model is a valuable model to study radiation and wound combined injury.

PMID:
21480768
PMCID:
PMC3193941
DOI:
10.3109/09553002.2011.568575
[Indexed for MEDLINE]
Free PMC Article
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