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Arch Virol. 2011 Sep;156(9):1603-6. doi: 10.1007/s00705-011-0997-9. Epub 2011 Apr 11.

Usefulness of a genotypic resistance test using dried blood spot specimens in African HIV-infected children with virological failure according to the 2010-revised WHO criteria.

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1
Laboratoire de Virologie, Assistance Publique-Hôpitaux de Paris (AP-HP), Hôpital Européen Georges Pompidou, Université Paris Descartes, Paris 5, 20 rue Leblanc, 75015 Paris, France. charlotte.charpentier@bch.aphp.fr

Abstract

We compared paired plasma and dried blood spot (DBS) samples from 54 HIV-1-treated children living in Bangui, Central African Republic, for antiretroviral-resistance-associated mutations. All children displayed virological failure (HIV-1 RNA >3.70 log(10)copies/ml). Testing for resistance genotype was carried out in a reference laboratory in Paris, France. A successful test result was obtained in 54 (100%) plasmas and 25 DBSs (46%). Among the 732 resistance-associated mutations analyzed, 718 were identical, leading to a high concordance rate of 98.1%. Genotypic resistance tests on DBS samples were found to be highly feasible and accurate in a foreign reference laboratory, but with additional costs for shipping and decreased sensitivity.

PMID:
21479946
DOI:
10.1007/s00705-011-0997-9
[Indexed for MEDLINE]

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