Format

Send to

Choose Destination
Mol Pharm. 2011 Jun 6;8(3):788-98. doi: 10.1021/mp100393j. Epub 2011 Apr 22.

Calcium condensed LABL-TAT complexes effectively target gene delivery to ICAM-1 expressing cells.

Author information

1
Department of Pharmaceutical Chemistry, The University of Kansas, Lawrence, Kansas 66047, USA.

Abstract

Targeted gene delivery using nonviral vectors is a highly touted scheme to reduce the potential for toxic or immunological side effects by reducing dose. In previous reports, TAT polyplexes with DNA have shown relatively poor gene delivery. The transfection efficiency has been enhanced by condensing TAT/DNA complexes to a small particle size using calcium. To explore the targetability of these condensed TAT complexes, LABL peptide targeting intercellular cell-adhesion molecule-1 (ICAM-1) was conjugated to TAT peptide using a polyethylene glycol (PEG) spacer. PEGylation reduced the transfection efficiency of TAT, but TAT complexes targeting ICAM-1 expressing cells regained much of the lost transfection efficiency. Targeted block peptides properly formulated with calcium offer promise for gene delivery to ICAM-1 expressing cells at sites of injury or inflammation.

PMID:
21473630
PMCID:
PMC4207658
DOI:
10.1021/mp100393j
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for American Chemical Society Icon for PubMed Central
Loading ...
Support Center