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J Am Soc Mass Spectrom. 2011 Jan;22(1):192-5. doi: 10.1007/s13361-010-0013-8. Epub 2011 Jan 20.

Matrix pre-coated MALDI MS targets for small molecule imaging in tissues.

Author information

1
Departments of Chemistry, Biochemistry, and the Mass Spectrometry Research Center, Vanderbilt University, MRB3, 465 21st Ave S, Nashville, TN 37235, USA.

Abstract

A new sample preparation method for MALDI tissue imaging has been developed for the analysis of low molecular weight compounds that employs matrix pre-coated MALDI targets. Tissue sections need only to be transferred onto the pre-coated target before analysis for fast and easy sample preparation. Pre-coated targets have a homogenous matrix coating with uniform crystals of approximately 1-2 μm and do not require solvents that may lead to analyte delocalization within a tissue section. We report here the use of matrix pre-coated targets for imaging of lipids, peptides, and pharmaceuticals in tissues.

PMID:
21472558
PMCID:
PMC4151471
DOI:
10.1007/s13361-010-0013-8
[Indexed for MEDLINE]
Free PMC Article

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