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Cell Physiol Biochem. 2011;27(3-4):313-26. doi: 10.1159/000327958. Epub 2011 Apr 1.

Alteration of the transcriptional profile of human embryonic kidney cells by transient overexpression of mouse TRPM7 channels.

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1
School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, Korea.

Abstract

BACKGROUND:

TRPM7 is a cation channel containing a functional kinase domain. The functional activity of TRPM7 is essential for cell viability and growth, and its expression is up-regulated in certain pathological conditions, such as ischemia.

METHODS:

In order to assess the effects of TRPM7 activity on cellular gene expression, inducible HEK293 cell-lines harboring the wild-type mouse TRPM7 and a mutant lacking the kinase domain were established. The wild-type and the non-functional TRPM7 channels were induced transiently and the comparative changes in cellular transcription were investigated.

RESULTS:

From the genome-scale analysis using a human genome expression microarray, we identified 951 genes altered in transcription significantly and specifically by the expression of the functional TRPM7 channel.

CONCLUSION:

By analyzing the genes differentially expressed by TRPM7, we were able to provide potential target proteins and to delineate the cellular pathways affected by the channel function.

PMID:
21471721
DOI:
10.1159/000327958
[Indexed for MEDLINE]
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