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J Gen Virol. 2011 Jul;92(Pt 7):1493-9. doi: 10.1099/vir.0.030601-0. Epub 2011 Apr 6.

Generation of neutralizing aptamers against herpes simplex virus type 2: potential components of multivalent microbicides.

Author information

1
Sir William Dunn School of Pathology, South Parks Road, Oxford OX1 3RE, UK. kenny.moore@path.ox.ac.uk

Abstract

The prophylactic use of topical antiviral agents has recently been validated by the reduction in human immunodeficiency virus (HIV) type 1 infection incidence seen using tonofovir-containing microbicides. In order to develop a wide-spectrum microbicide to prevent infection with a wide range of sexually transmitted viruses, we have previously reported the development of HIV-neutralizing aptamers and here report the isolation and characterization of aptamers that neutralize herpes simplex virus type 2 (HSV-2). These aptamers bind the envelope glycoprotein (gD), are potent (IC(50) of 20-50 nM) and are able to block infection pathways dependent on both major entry receptors, Nectin1 and HVEM. Structural analysis and mutagenesis of these aptamers reveal a core specificity element that could provide the basis for pharmaceutical development. As HSV-2 is a major risk factor for the acquisition of HIV-1, a microbicide capable of preventing HSV-2 infection would not only reduce the morbidity associated with HSV-2, but also that derived from HIV-1.

PMID:
21471320
PMCID:
PMC3167898
DOI:
10.1099/vir.0.030601-0
[Indexed for MEDLINE]
Free PMC Article

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