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Br J Cancer. 2011 Apr 26;104(9):1410-7. doi: 10.1038/bjc.2011.94. Epub 2011 Apr 5.

Downregulation of transcription factor SOX2 in cancer stem cells suppresses growth and metastasis of lung cancer.

Author information

1
Department of Immunology and Microbial Science, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.

Erratum in

  • Br J Cancer. 2011 Jun 7;104(12):1931.

Abstract

BACKGROUND:

The cancer stem cell hypothesis suggests that neoplastic clones are maintained exclusively by a small subpopulation of cells, which have indefinite proliferation and differentiation potentials and give rise to phenotypically diverse cancer cells. Cancer stem cells have been isolated by their ability to efflux Hoechst 33342 dye and are referred to as the 'side population' (SP).

METHODS AND RESULTS:

The Hoechst efflux assay was used to isolate and characterize the SP from murine D121 lung carcinoma cells. Here, we demonstrated that D121-SP cells contain cancer stem cell characteristics, that is, upregulation of the transcription factors SOX2 and Oct 4 in D121-SP cells. In addition, the migration of D121-SP was decreased, and apoptosis of D121-SP was upregulated following knocking down of SOX2 in D121 cells. Importantly, downregulation of SOX2 in D121 cells markedly suppressed their metastatic potential in syngeneic mice.

CONCLUSIONS:

These results suggest that the SP is an enriched source of lung tumour cells with stem cell properties and that SOX2 has an important role in maintaining stem cell properties and functions that may be a potential target for effective lung cancer therapy.

PMID:
21468047
PMCID:
PMC3101944
DOI:
10.1038/bjc.2011.94
[Indexed for MEDLINE]
Free PMC Article

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