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Sci Signal. 2011 Apr 5;4(167):pt2. doi: 10.1126/scisignal.2001827.

Proteomic analysis of integrin adhesion complexes.

Author information

1
Wellcome Trust Centre for Cell-Matrix Research, University of Manchester, UK.

Abstract

Integrin receptors regulate cell fate by coupling the binding of extracellular adhesion proteins to the assembly of intracellular cytoskeletal and signaling complexes. A detailed, integrative view of adhesion complexes will provide insight into the molecular mechanisms that control cell morphology, survival, movement, and differentiation. To date, membrane receptor-associated signaling complexes have been refractory to proteomic analysis because of their inherent lability and inaccessibility. We developed a methodology to isolate ligand-induced integrin adhesion complexes, and we used this technique to analyze the composition of complexes associated with multiple receptor-ligand pairs and define core and receptor-specific subnetworks. In particular, we identified regulator of chromosome condensation-2 (RCC2) as a component of fibronectin-activated signaling pathways that regulate directional cell movement. The development of this proteomics pipeline provides the means to investigate the molecular composition and function of various adhesion complexes.

PMID:
21467297
DOI:
10.1126/scisignal.2001827
[Indexed for MEDLINE]

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