Format

Send to

Choose Destination
Methods Mol Biol. 2011;735:47-54. doi: 10.1007/978-1-61779-092-8_5.

Telomere G-overhang length measurement method 1: the DSN method.

Author information

1
Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA.

Abstract

Telomeres terminate in 3' single-stranded G-overhangs that function in telomere end protection and telomerase action. An accurate measurement of overhang length is challenging due to the presence of many kilobases of double-stranded telomere DNA. Here, a simple method is described that utilizes duplex-specific nuclease (DSN) to digest all genomic DNA including telomeres, leaving the single-stranded overhangs intact. The telomere single-strand G-rich overhang length can then be determined by Southern blot-based assays.

PMID:
21461810
PMCID:
PMC3528100
DOI:
10.1007/978-1-61779-092-8_5
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Springer Icon for PubMed Central
Loading ...
Support Center