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FEBS Lett. 1990 Oct 15;272(1-2):125-7.

Comparative properties of human alpha-1-proteinase inhibitor glycosylation variants.

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1
Institute of Molecular Biology, Jagiellonian University, Krakow, Poland.

Abstract

Variant forms of human alpha-1-proteinase inhibitor (alpha-1-PI), obtained by the treatment of human Hep G2 cells with specific inhibitors of glycosylation were tested for both inhibitory activity and heat stability. All were found to have the same second-order association rate with human neutrophil elastase, indicating a lack of importance of the carbohydrate moiety. In contrast, incompletely glycosylated forms of alpha-1-PI were found to be heat sensitive relative to the mature protein, suggesting a role for carbohydrate in protein stabilization.

PMID:
2146158
DOI:
10.1016/0014-5793(90)80464-t
[Indexed for MEDLINE]
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