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Theranostics. 2011;1:18-27. Epub 2011 Jan 12.

Real-time video imaging of protease expression in vivo.

Author information

1
1. Laboratory of Molecular Imaging and Nanomedicine, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland 20892, USA.

Abstract

We demonstrate the first true real-time in vivo video imaging of extracellular protease expression using an ultrafast-acting and extended-use activatable probe. This simple, one-step technique is capable of boosting fluorescent signals upon target protease cleavage as early as 30 minutes from injection in a small animal model and is able to sustain the strong fluorescent signal up to 24 hours. Using this method, we video imaged the expression and inhibition of matrix metalloproteinases (MMPs) in a tumor-bearing mouse model. The current platform can be universally applied to any target protease of interest with a known peptide substrate and is adaptable to a wide range of real-time imaging applications with high throughputs such as for in vivo drug screening, examinations of the therapeutic efficacy of drugs, and monitoring of disease onset and development in animal models.

KEYWORDS:

PEGylation.; activatable probes; matrix metalloproteinases (MMPs); near-infrared fluorescence imaging; peptide substrate; real-time in vivo video imaging

PMID:
21461134
PMCID:
PMC3068198

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