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Nat Methods. 2011 May;8(5):405-7. doi: 10.1038/nmeth.1592. Epub 2011 Apr 3.

A genome-scale shRNA resource for transgenic RNAi in Drosophila.

Author information

1
Department of Genetics, Harvard Medical School, Howard Hughes Medical Institute, Boston, Massachusetts, USA.

Abstract

Existing transgenic RNAi resources in Drosophila melanogaster based on long double-stranded hairpin RNAs are powerful tools for functional studies, but they are ineffective in gene knockdown during oogenesis, an important model system for the study of many biological questions. We show that shRNAs, modeled on an endogenous microRNA, are extremely effective at silencing gene expression during oogenesis. We also describe our progress toward building a genome-wide shRNA resource.

PMID:
21460824
PMCID:
PMC3489273
DOI:
10.1038/nmeth.1592
[Indexed for MEDLINE]
Free PMC Article

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