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Nat Methods. 2011 May;8(5):409-12. doi: 10.1038/nmeth.1591. Epub 2011 Apr 3.

A more efficient method to generate integration-free human iPS cells.

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1
Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan. okita@cira.kyoto-u.ac.jp

Abstract

We report a simple method, using p53 suppression and nontransforming L-Myc, to generate human induced pluripotent stem cells (iPSCs) with episomal plasmid vectors. We generated human iPSCs from multiple donors, including two putative human leukocyte antigen (HLA)-homozygous donors who match ∼20% of the Japanese population at major HLA loci; most iPSCs are integrated transgene-free. This method may provide iPSCs suitable for autologous and allologous stem-cell therapy in the future.

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PMID:
21460823
DOI:
10.1038/nmeth.1591
[Indexed for MEDLINE]

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