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Biochem Pharmacol. 2011 Jul 1;82(1):17-23. doi: 10.1016/j.bcp.2011.03.017. Epub 2011 Mar 29.

Dendrimers-delivered short hairpin RNA targeting hTERT inhibits oral cancer cell growth in vitro and in vivo.

Author information

1
Department of Oral and Maxillofacial Surgery, Guanghua School of Stomatology & Institute of Stomatological Research, Sun Yat-sen University, Guangzhou, Guangdong, China. xiqiangliu@yahoo.com.cn

Abstract

Promising therapeutic application of RNA interference (RNAi) depends on the availability of safe and efficient intracellular delivery systems. Human telomerase reverse transcriptase (hTERT), the catalytic subunit of telomerase complex, is an attractive therapeutic target for oral cancer. Here we investigated the characteristics and anticancer effect of polyamidoamine (PAMAM) dendrimer-mediated short hairpin RNA (shRNA) against hTERT in oral cancer. Dendrimer-mediated shRNA efficiently silenced the hTERT gene in vitro, resulted in cell growth inhibition and apoptosis. Treatment with the shRNA dendriplex attenuated tumor growth in a xenograft model. These studies suggest that RNAi-mediated hTERT gene silencing, coupled with dendrimer delivery, may provide a promising approach for the treatment of oral cancer, in which hTERT is abundantly expressed.

PMID:
21453684
DOI:
10.1016/j.bcp.2011.03.017
[Indexed for MEDLINE]

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