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J Am Chem Soc. 2011 Apr 20;133(15):5698-700. doi: 10.1021/ja200444t. Epub 2011 Mar 30.

A mitochondrial-targeted two-photon probe for zinc ion.

Author information

1
Molecular Science and Technology Research Center, Ajou University, Suwon 443-749, Korea.

Abstract

We report a two-photon probe (SZn-Mito) for mitochondrial zinc ions ([Zn2+]m). This probe shows a 7-fold enhancement of two-photon-excited fluorescence in response to Zn2+ with a dissociation constant (Kd(TP)) of 3.1 ± 0.1 nM and pH insensitivity in the biologically relevant range, allowing the detection of [Zn2+]m in a rat hippocampal slice at a depth of 100−200 μm without interference from other metal ions through the use of two-photon microscopy.

PMID:
21449534
DOI:
10.1021/ja200444t
[Indexed for MEDLINE]

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