Format

Send to

Choose Destination
Genomics. 2011 Jul;98(1):26-39. doi: 10.1016/j.ygeno.2011.03.008. Epub 2011 Apr 5.

Investigating the genome diversity of B. cereus and evolutionary aspects of B. anthracis emergence.

Author information

1
Pathogen Functional Genomics Resource Center (PFGRC), The J. Craig Venter Institute (JCVI), Rockville, MD 20850, USA.

Abstract

Here we report the use of a multi-genome DNA microarray to investigate the genome diversity of Bacillus cereus group members and elucidate the events associated with the emergence of Bacillus anthracis the causative agent of anthrax-a lethal zoonotic disease. We initially performed directed genome sequencing of seven diverse B. cereus strains to identify novel sequences encoded in those genomes. The novel genes identified, combined with those publicly available, allowed the design of a "species" DNA microarray. Comparative genomic hybridization analyses of 41 strains indicate that substantial heterogeneity exists with respect to the genes comprising functional role categories. While the acquisition of the plasmid-encoded pathogenicity island (pXO1) and capsule genes (pXO2) represents a crucial landmark dictating the emergence of B. anthracis, the evolution of this species and its close relatives was associated with an overall shift in the fraction of genes devoted to energy metabolism, cellular processes, transport, as well as virulence.

PMID:
21447378
PMCID:
PMC3129444
DOI:
10.1016/j.ygeno.2011.03.008
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center