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J Biophotonics. 2011 Jun;4(6):435-41. doi: 10.1002/jbio.201000120. Epub 2011 Mar 28.

Fast and long term lipid droplet tracking with CARS microscopy.

Author information

1
Departement Chemie and Center for Applied Photonics, Universität Konstanz, 78457 Konstanz, Germany.

Abstract

Photobleaching of organic fluorophores commonly used in fluorescence microscopy puts a limit to the number of images which can be acquired. Label-free imaging techniques therefore offer advantages both for rapid image acquisition and for long-term observations. CARS microscopy is a label-free imaging technique offering molecule specific contrast. Here we demonstrate that CARS microscopy allows video-rate tracking of intracellular transport of lipid droplets, but also continuous long-term observation of cells over several hours.

PMID:
21445955
DOI:
10.1002/jbio.201000120
[Indexed for MEDLINE]

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