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J Am Chem Soc. 2011 Apr 27;133(16):6102-5. doi: 10.1021/ja108404p. Epub 2011 Mar 28.

Imaging of EdU, an alkyne-tagged cell proliferation probe, by Raman microscopy.

Author information

1
Sodeoka Live Cell Chemistry Project, ERATO, Japan Science and Technology Agency, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.

Abstract

Click-free imaging of the nuclear localization of an alkyne-tagged cell proliferation probe, EdU, in living cells was achieved for the first time by means of Raman microscopy. The alkyne tag shows an intense Raman band in a cellular Raman-silent region that is free of interference from endogenous molecules. This approach may eliminate the need for click reactions in the detection of alkyne-labeled molecules.

PMID:
21443184
DOI:
10.1021/ja108404p
[Indexed for MEDLINE]

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