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Arch Virol. 2011 Aug;156(8):1425-9. doi: 10.1007/s00705-011-0973-4. Epub 2011 Mar 26.

Molecular characterization of the RNA-dependent RNA polymerase from groundnut ringspot virus (genus Tospovirus, family Bunyaviridae).

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1
Laboratory of Plant Virology, Department of Cellular Biology, Biological Sciences Institute, University of Brasília, Campus Universitário Darcy Ribeiro, Asa Norte, Brasília, Distrito Federal, 70910-900, Brazil.

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  • Arch Virol. 2011 Aug;156(8):1431-2.

Abstract

Groundnut ringspot virus is a negative-sense single-stranded RNA virus that belongs to the genus Tospovirus and is the prevalent member of this genus in Brazil. This work presents the nucleotide sequence of the L RNA, with a single open reading frame of 2873 amino acids in the complementary strand corresponding to the RNA-dependent RNA polymerase (L protein), as well as the characterization of conserved domains of the L protein by in silico analysis. Phylogenetic analysis of different L protein domains confirmed that GRSV is a member of the American clade, and comparison with a N-protein indicates that phylogeny based on L protein sequences may be more reliable than that based on the N protein.

PMID:
21442231
DOI:
10.1007/s00705-011-0973-4
[Indexed for MEDLINE]
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