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J Virol Methods. 2011 Jun;174(1-2):128-30. doi: 10.1016/j.jviromet.2011.03.020. Epub 2011 Apr 2.

Poliovirus serotype-specific VP1 sequencing primers.

Author information

1
Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA. DKilpatrick@cdc.gov

Abstract

The Global Polio Laboratory Network routinely uses poliovirus-specific PCR primers and probes to determine the serotype and genotype of poliovirus isolates obtained as part of global poliovirus surveillance. To provide detailed molecular epidemiologic information, poliovirus isolates are further characterized by sequencing the ~900-nucleotide region encoding the major capsid protein, VP1. It is difficult to obtain quality sequence information when clinical or environmental samples contain poliovirus mixtures. As an alternative to conventional methods for resolving poliovirus mixtures, sets of serotype-specific primers were developed for amplifying and sequencing the VP1 regions of individual components of mixed populations of vaccine-vaccine, vaccine-wild, and wild-wild polioviruses.

PMID:
21440569
DOI:
10.1016/j.jviromet.2011.03.020
[Indexed for MEDLINE]

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