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Curr Opin Biotechnol. 2011 Jun;22(3):465-72. doi: 10.1016/j.copbio.2011.02.010. Epub 2011 Mar 24.

The art and design of functional metagenomic screens.

Author information

1
Department of Microbiology & Immunology, University of British Columbia, Canada.

Abstract

This article summarizes general design principles for functional metagenomics. The focus is on Escherichia coli as an expression host, although alternative host-vector systems are discussed in relation to optimizing gene recovery in activity-based screens. Examples of DNA isolation and enrichment approaches, library construction and phenotypic read-out are described with special emphasis on the use of high throughput technologies for rapid isolation of environmental clones encoding phenotypic traits of interest.

PMID:
21440432
DOI:
10.1016/j.copbio.2011.02.010
[Indexed for MEDLINE]

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