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Lab Chip. 2011 May 7;11(9):1593-5. doi: 10.1039/c1lc20084d. Epub 2011 Mar 24.

How to embed three-dimensional flexible electrodes in microfluidic devices for cell culture applications.

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Bioengineering Department, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano, Italy.


This communication describes a simple, rapid and cost effective method of embedding a conductive and flexible material within microfluidic devices as a means to realize uniform electric fields within cellular microenvironments. Fluidic channels and electrodes are fabricated by traditional soft-lithography in conjunction with chemical etching of PDMS. Devices can be deformable (thus allowing for a combination of electro-mechanical stimulation), they are made from inexpensive materials and easily assembled by hand; this method is thus accessible to a wide range of laboratories and budgets.

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