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Neuron. 2011 Mar 24;69(6):1114-31. doi: 10.1016/j.neuron.2011.02.007.

Hts/Adducin controls synaptic elaboration and elimination.

Author information

1
Department of Biochemistry and Biophysics, University of California, San Francisco, 1550 4(th) Street, San Francisco, CA 94158, USA. jan.pielage@fmi.ch

Abstract

Neural development requires both synapse elaboration and elimination, yet relatively little is known about how these opposing activities are coordinated. Here, we provide evidence Hts/Adducin can serve this function. We show that Drosophila Hts/Adducin is enriched both pre- and postsynaptically at the NMJ. We then demonstrate that presynaptic Hts/Adducin is necessary and sufficient to control two opposing processes associated with synapse remodeling: (1) synapse stabilization as determined by light level and ultrastructural and electrophysiological assays and (2) the elaboration of actin-based, filopodia-like protrusions that drive synaptogenesis and growth. Synapse remodeling is sensitive to Hts/Adducin levels, and we provide evidence that the synaptic localization of Hts/Adducin is controlled via phosphorylation. Mechanistically, Drosophila Hts/Adducin protein has actin-capping activity. We propose that phosphorylation-dependent regulation of Hts/Adducin controls the level, localization, and activity of Hts/Adducin, influencing actin-based synapse elaboration and spectrin-based synapse stabilization. Hts/Adducin may define a mechanism to switch between synapse stability and dynamics.

PMID:
21435557
PMCID:
PMC3073818
DOI:
10.1016/j.neuron.2011.02.007
[Indexed for MEDLINE]
Free PMC Article

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