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Insect Mol Biol. 2011 Jun;20(3):409-15. doi: 10.1111/j.1365-2583.2011.01075.x. Epub 2011 Mar 24.

Causal involvement of mammalian-type cryptochrome in the circadian cuticle deposition rhythm in the bean bug Riptortus pedestris.

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1
Graduate School of Science, Osaka City University, Osaka, Japan Institute of Low Temperature Science, Hokkaido University, Sapporo, Japan Graduate School of Science, Kyoto University, Kyoto, Japan.

Abstract

Mammalian-type CRYPTOCHROME (CRY-m) is considered to be a core repressive component of the circadian clock in various insect species. However, this role is based only on the molecular function of CRY-m in cultured cells and it therefore remains unknown whether CRY-m is indispensable for governing physiological rhythms at the organismal level. In the present study, we show that RNA interference (RNAi) targeting of cry-m in the bean bug Riptortus pedestris disrupts the circadian clock governing the cuticle deposition rhythm and results in the generation of a single cuticle layer. Furthermore, period expression was induced in cry-m RNAi insects. These results verified that CRY-m functions as a negative regulator in the circadian clock that generates physiological rhythm at the organismal level.

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