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Methods Mol Biol. 2011;714:407-22. doi: 10.1007/978-1-61779-005-8_25.

RIP: an mRNA localization technique.

Author information

1
Department of Nanobioscience, University at Albany, Albany, NY, USA.

Abstract

A detailed understanding of post-transcriptional gene expression is necessary to correlate the different elements involved in the many levels of RNA-protein interactions that are needed to coordinate the cellular biomolecular machinery. The profile of mRNA, a major component of this machinery, can be examined after isolation from specific RNA-binding proteins (RBPs). RIP-Chip or ribonomic profiling is a versatilein vivo technique that has been widely used to study post-transcriptional gene regulation and the localization of mRNA. Here we elaborately detail the methodology for mRNA isolation using RBP immunoprecipitation (RIP) as a primary approach. Specific antibodies are used to target RBPs, which are then used to capture the associated mRNA.

PMID:
21431755
DOI:
10.1007/978-1-61779-005-8_25
[Indexed for MEDLINE]

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