Format

Send to

Choose Destination
Methods Mol Biol. 2011;721:183-98. doi: 10.1007/978-1-61779-037-9_11.

Characterization of RISC-associated adenoviral small RNAs.

Author information

1
Department of Medical Biochemistry and Microbiology, Uppsala Biomedical Center (BMC), Uppsala, Sweden.

Abstract

RNA interference (RNAi) plays novel roles in both host antiviral defense and viral replication. It has been shown that some viruses can exploit the RNAi machinery for their own benefit by encoding for their own viral small RNAs. Here we present a collection of methods to study adenoviral small RNAs, specifically a method for immunopurification of RNA-induced silencing complex (RISC) and a biochemical assay for the activity of purified RISC associated with adenoviral small RNAs.

PMID:
21431686
DOI:
10.1007/978-1-61779-037-9_11
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center