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Methods Mol Biol. 2011;721:107-22. doi: 10.1007/978-1-61779-037-9_6.

Viral small RNA cloning and sequencing.

Author information

1
CNRS URA 3015, Institut Pasteur, Viruses and RNAi group, Paris, France.

Abstract

At the current rate of technological progress, high-throughput sequencing of nucleic acids has become a commodity. These techniques are perfectly suitable for viral small RNAs sequencing and contribute to the understanding of many aspects of virus biology in the context of host-pathogen interaction. However, the generation of high quality data is still an issue and the preparation of small RNAs libraries that accurately reflect the viral siRNAs in the sample remains a challenge. In this chapter we describe how to clone and sequence libraries of viral small RNAs from infected insect samples (mosquito, drosophilidae, insect-derived cell lines).

PMID:
21431681
DOI:
10.1007/978-1-61779-037-9_6
[Indexed for MEDLINE]

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