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J Clin Microbiol. 2011 May;49(5):2031-4. doi: 10.1128/JCM.00615-10. Epub 2011 Mar 23.

Bacterial identification by 16S rRNA gene PCR-hybridization as a supplement to negative culture results.

Author information

1
Division of Infectious Disease, Children's Hospital Los Angeles, and Metic Transplantation Laboratory,Keck School of Medicine, University of Southern California, Los Angeles, California, USA. kmatsuda@usc.edu.

Abstract

PCR-hybridization was compared to culture methods for evaluating suspected blood infections. A total of 231 clinical samples from blood culture bottles that were flagged positive by the BacT/Alert system or were negative 1 week after inoculation were tested. When the PCR-hybridization and culture method results were compared, the positive and negative concordance rates were 99.2% (122/123) and 89.5% (94/105), respectively. Of the negative blood cultures, 10.5% (11/105) were positive by PCR-hybridization. Supplemental testing of negative blood cultures may identify bacterial pathogens that are undetectable by culture methods.

PMID:
21430102
PMCID:
PMC3122699
DOI:
10.1128/JCM.00615-10
[Indexed for MEDLINE]
Free PMC Article

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