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Anal Chem. 2011 Apr 15;83(8):3192-8. doi: 10.1021/ac103093w. Epub 2011 Mar 23.

Improved LC-MS method for the determination of fatty acids in red blood cells by LC-orbitrap MS.

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Cancer Research Center of Hawaii, 1236 Lauhala Street, Honolulu, Hawaii 96813, USA.


We report a new method for fast and sensitive analyses of biologically relevant fatty acids (FAs) in red blood cells (RBC) by liquid chromatography mass spectrometry (LC-MS). A new chemical derivatization approach was developed forming picolylamides from FAs in a quantitative reaction. Fourteen derivatized FA standards, including saturated and unsaturated FAs from C14 to C22, were efficiently separated within 15 min. In addition, the use of a recently introduced benchtop orbitrap mass spectrometer under positive electrospray ionization (ESI) full scan mode showed a 2-10-fold improvement in sensitivity compared with a conventional tandem MS method, with a limit of detection in the low femtomole range for saturated and unsaturated FAs. The developed method was applied to determine FA concentrations in RBC with intra- and interday coefficients of variation below 10%.

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