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Fa Yi Xue Za Zhi. 2010 Dec;26(6):446-8.

[SNP typing of formalin-fixed tissue in individual identification].

[Article in Chinese]

Author information

1
Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, PR China, Shanghai 200063, China. liny@ssfjd.cn

Abstract

OBJECTIVE:

To explore the method for DNA typing in formalin-fixed tissue by detecting SNP markers on X chromosome.

METHODS:

Genomic DNA was prepared from formalin-fixed tissue. In the event that typing using Sinofiler and MiniFiler kits had failed, 51 SNPs were amplified with multiplex PCR and typed with matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). RESULTS Full profiles of X-SNP loci were obtained from the formalin-fixed tissue, while the STR and miniSTR genotyping failed.

CONCLUSION:

SNP genotyping technique can be used to obtain more information for formalin fixed tissues.

PMID:
21425609
[Indexed for MEDLINE]
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