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Mol Biol Rep. 2012 Jan;39(1):13-6. doi: 10.1007/s11033-011-0704-7. Epub 2011 Mar 22.

Evaluation of DRB1 high resolution typing by a new SSO-based Luminex method.

Author information

1
IME Foundation, Department of Laboratory Medicine, PTV-Hospital University Tor Vergata Rome, Rome, Italy.

Abstract

HLA testing is an essential part of the process to identify a donor who may be a good match for the patients who need haematopoietic stem cells from bone marrow, peripheral blood or cord blood and the DNA typing in high resolution is now recommended as the Scientific Societies also describe in their standards. Recently the new PCR-Luminex HLA typing method, based on the reverse sequence specific oligonucleotide probes coupled with a microsphere beads in an array platform, has been well established. We report the data from 146 samples previously typed to a four digits level and used to evaluate the accuracy, sensitivity and performance of the new high definition DRB1 by PCR-Luminex kit. One hundred and forty-six samples from unrelated healthy donors, haematological patients or external proficiency tests were used in this study. The Luminex high definition DRB1 typing represents a versatile method and may be easily introduced in the routine, particularly when the technical team has already acquired experience on the technique. Only few HLA allelic combinations need an additional typing by PCR-SSP or SBT to solve the ambiguous results thus reducing the time necessary to produce a final report.

PMID:
21424786
DOI:
10.1007/s11033-011-0704-7
[Indexed for MEDLINE]

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