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[The effect of CTLA4-Ig gene modified dendritic cell on Th1/Th2 balance in vitro].

[Article in Chinese]

Author information

1
Department of Respiratory Center, Children's Hospital, Chongqing Medical University, Chongqing 400014, China. yinglinyan2006@yahoo.com.cn

Abstract

AIM:

To investigate the impact on Th1/Th2 subset balance from cytotoxic T lymphocyte-associated antigen immunoglobulin (CTLA4 Ig) gene-modified dendritic cells (CTLA4 Ig-DCs) in vitro.

METHODS:

The modified DCs (CTIA4 Ig-DCs) were prepared by transferring the mouse bone marrow-derived DCs with the constructed adenovirus CTIA4 Ig vectorî The CTLA4 Ig expression and certain cell surface molecules on the CTIA4Ig-DCs were detected by FCM, the potential to stimulate allogeneic T cell proliferation of the modified DCs by mixed lymphocyte reaction (MLR) and the secretion of IFN-γ and IL-4 in antigen presentation by ELISA.

RESULTS:

CTLA4 Ig gene was successfully transfected into DCs with stable expression of CTLA4 Ig, and its transfection efficiency was about 80%. Compared with the controls, CTLA4 Ig-DCs showed lower surface molecule CD86, inhibited allogeneic lymphocyte proliferation in MLR, decreased secretion of IFN-γ and IL-4 and increased ratio of IFN-γ/IL-4 in antigen presentation.

CONCLUSION:

CTLA4 Ig-DCs were successfully obtained and effectively expressed CTLA4 Ig, which could reduce the expression of CD86 on DCs surface. CTLA4 Ig-DCs inhibited allogeneic T cell proliferation and affected the ratio of Th1/Th2 in vitro.

PMID:
21419045
[Indexed for MEDLINE]
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