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Analysis of Chromatin Assembly, Chromatin Domains, and DNA Replication Using Xenopus Systems.

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Mapping Protein/DNA Interactions by Cross-Linking [Internet]. Paris: Institut national de la santé et de la recherche médicale; 2001.

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We will describe in the next sections the preparation of cell-free extracts from eggs or early embryos and their use in chromatin assembly chromatin binding and DNA replication in vitro. These protocols as well as other related protocols related to the use of Xenopus eggs and embryos are reported inMenut et al. (1999) and available at www.igh.cnrs.fr./equip/mechali. We will also describe here the use of nuclear matrix purification as a tool to analyze chromatin domains involved in gene regulation. This method was applied with success both in Xenopus embryos and adult tissues (Vassetzky et al. 2000).

Copyright © 2001, Institut national de la santé et de la recherche médicale (INSERM).

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