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Faraday Discuss. 2011;149:23-36; discussion 63-77.

Development of reagents and assays for the detection of pathogenic Burkholderia species.

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Institute for Cellular and Molecular Biology, The University of Texas at Austin, 1 University Station A4800, Austin, Texas 78712, USA.


Rapid detection of the category B biothreat agents Burkholderia pseudomallei and Burkholderia mallei in acute infections is critical to ensure that appropriate treatment is administered quickly to reduce an otherwise high probability of mortality (ca. 40% for B. pseudomallei). We are developing assays that can be used in clinical laboratories or security applications for the direct detection of surface-localized and secreted macromolecules produced by these organisms. We present our current medium-throughout approach for target selection and production of Burkholderia macromolecules and describe the generation of a Fab molecule targeted to the B. mallei BimA protein. We also present development of prototype assays for detecting Burkholderia species using anti-lipopolysaccharide antibodies.

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