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Hum Exp Toxicol. 2011 Dec;30(12):1931-44. doi: 10.1177/0960327111402242. Epub 2011 Mar 15.

Protective effect of Glycine max and Chrysanthemum indicum extracts against cisplatin-induced renal epithelial cell death.

Author information

1
Department of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.

Abstract

Although cisplatin is one of the most efficient chemotherapeutic agents for the treatment of solid tumors, frequently observed nephrotoxicity has limited its use in several patients.

MATERIALS AND METHODS:

The protective effect of Glycine max (GM) and Chrysanthemum indicum (CM) extracts on cisplatin-induced apoptosis in human proximal tubular HK-2 cells was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Hoechst 33342, and propidium iodide assays. Reactive oxygen species (ROS) were determined by flow cytometry with 2,7-dichlorofluorescein diacetate (DCFH(2)-DA).

RESULTS:

Cisplatin-induced renal cell toxicity through the induction of hydrogen peroxide (H(2)O(2)) and hydroxyl radical (OH(•-)). CM extract protected cisplatin-induced apoptosis by its anti-oxidant activity against H(2)O(2) and OH(•-), while GM extract scavenged only H(2)O(2). Furthermore, GM and CM extracts protect renal cells without significant interfering effect on cisplatin toxicity in lung cancer H460 and melanoma G361 cells.

CONCLUSION:

GM and CM extracts exhibited a promising protective effect on cisplatin-induced nephrotoxicity which could benefit the development for nephroprotective approaches.

PMID:
21406484
DOI:
10.1177/0960327111402242
[Indexed for MEDLINE]

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