Format

Send to

Choose Destination
See comment in PubMed Commons below
Curr Protoc Protein Sci. 2011 Feb;Chapter 12:Unit12.9. doi: 10.1002/0471140864.ps1209s63.

High-throughput lectin microarray-based analysis of live cell surface glycosylation.

Author information

1
Department of Pathology, Oncology & Medicine and Institute for Cell Engineering, Johns Hopkins University, Baltimore, Maryland, USA.

Abstract

Lectins, plant-derived glycan-binding proteins, have long been used to detect glycans on cell surfaces. However, the techniques used to characterize serum or cells have largely been limited to mass spectrometry, blots, flow cytometry, and immunohistochemistry. While these lectin-based approaches are well established and they can discriminate a limited number of sugar isomers by concurrently using a limited number of lectins, they are not amenable for adaptation to a high-throughput platform. Fortunately, given the commercial availability of lectins with a variety of glycan specificities, lectins can be printed on a glass substrate in a microarray format to profile accessible cell-surface glycans. This method is an inviting alternative for analysis of a broad range of glycans in a high-throughput fashion and has been demonstrated to be a feasible method of identifying binding-accessible cell surface glycosylation on living cells. The current unit presents a lectin-based microarray approach for analyzing cell surface glycosylation in a high-throughput fashion.

PMID:
21400689
PMCID:
PMC3090205
DOI:
10.1002/0471140864.ps1209s63
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Wiley Icon for PubMed Central
    Loading ...
    Support Center