Format

Send to

Choose Destination
Curr Protoc Microbiol. 2011 Feb;Chapter 1:Unit 1E.4. doi: 10.1002/9780471729259.mc01e04s20.

Preparing DNA libraries for multiplexed paired-end deep sequencing for Illumina GA sequencers.

Author information

1
Dartmouth Medical School, Hanover, New Hampshire, USA.

Abstract

Whole-genome sequencing, also known as deep sequencing, is becoming a more affordable and efficient way to identify SNP mutations, deletions, and insertions in DNA sequences across several different strains. Two major obstacles preventing the widespread use of deep sequencers are the costs involved in services used to prepare DNA libraries for sequencing and the overall accuracy of the sequencing data. This unit describes the preparation of DNA libraries for multiplexed paired-end sequencing using the Illumina GA series sequencer. Self-preparation of DNA libraries can help reduce overall expenses, especially if optimization is required for the different samples, and use of the Illumina GA Sequencer can improve the quality of the data.

PMID:
21400673
PMCID:
PMC3076644
DOI:
10.1002/9780471729259.mc01e04s20
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Wiley Icon for PubMed Central
Loading ...
Support Center