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Methods Mol Biol. 1988;3:383-93. doi: 10.1385/0-89603-126-8:383.

Vacuum blotting : an inexpensive, flexible, qualitative blotting technique.

Author information

1
St. Raphael, Laboratory of Hematology, University Hospital, Leuven, Belgium.

Abstract

The first system we used to transfer proteins from polyacrylamide gels onto nitrocellulose was a capillary blotting system, based on the work of Renart et al. (1). Although we were very enthusiastic about the wide range of possible applications created by the protein blotting technique, we were unsatisfied with the slow and inefficient transfer. Electroblotting (2,3) seemed to be much more efficient, but at that time there were serious financial limitations in the department.

PMID:
21400177
DOI:
10.1385/0-89603-126-8:383

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