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Methods Mol Biol. 1988;3:25-32. doi: 10.1385/0-89603-126-8:25.

The bradford method for protein quantitation.

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1
Biochemistry Department, Rothamsted Experimental Station, Hertfordshire, UK.

Abstract

A rapid and accurate method for the estimation of protein concentration is essential in many fields of protein study. The Lowry method ( Chapter 1 in vol. 1 of this series) has been widely used, but is susceptible to interference from a wide range of compounds commonly present in biological extracts. Although interference can be avoided by trichloracetic acid precipitation of the protein prior to assay, this lengthens the procedure.

PMID:
21400151
DOI:
10.1385/0-89603-126-8:25

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