Format

Send to

Choose Destination
Br J Ophthalmol. 2011 Jul;95(7):1006-9. doi: 10.1136/bjo.2010.194571. Epub 2011 Mar 11.

Enhancement of corneal endothelium wound healing by Rho-associated kinase (ROCK) inhibitor eye drops.

Author information

1
Department of Biomedical Engineering, Faculty of Life and Medical Sciences, Doshisha University, Tatara, Kyotanabe 610-0321, Japan. nkoizumi@mail.doshisha.ac.jp

Abstract

AIM:

To demonstrate the efficacy of Rho-associated kinase (ROCK) inhibitor Y-27632 for corneal endothelial wound healing both in in vitro and in vivo models.

METHODS:

As an in vitro model, cultivated cynomolgus monkey corneal endothelial cells were scraped to create a linear defect. The wound distance was then determined during a 24-h culture in the presence or absence of 10 μM of Y-27632. As an in vivo model, central corneal endothelium of Japanese white rabbits was damaged by transcorneal freezing, then 10 mM of Y-27632 was applied topically six times daily for 48 h. The wound area of the corneal endothelium was evaluated after 48 h.

RESULTS:

The mean wound distance in the cultured corneal endothelial cells was significantly shorter in the Y-27632 group than in the control group. In the rabbit model, the mean wound area of the Y-27632 group was significantly smaller than that of the control group.

CONCLUSION:

This study demonstrated that ROCK inhibitor Y-27632 promotes corneal endothelial wound healing both in in vitro and in vivo.

PMID:
21398412
DOI:
10.1136/bjo.2010.194571
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for HighWire
Loading ...
Support Center