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Diagn Microbiol Infect Dis. 2011 Aug;70(4):452-4. doi: 10.1016/j.diagmicrobio.2010.09.006. Epub 2011 Mar 12.

Use of 2 pneumococcal common protein real-time polymerase chain reaction assays in healthy children colonized with Streptococcus pneumoniae.

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Centers for Diseases Control and Prevention, Atlanta, GA 30329, USA.


Polymerase chain reaction (PCR) offers promise in pneumococcal diagnostics, but whether nasopharyngeal carriage causes false-positive results in people colonized with Streptococcus pneumoniae is unknown. We found in serum no positive samples for pneumococcal DNA in 100 carriers and noncarriers using 2 different real-time PCR assays targeting lytA and psaA genes.

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