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ACS Nano. 2011 Apr 26;5(4):3166-71. doi: 10.1021/nn2003053. Epub 2011 Mar 17.

Selective chromogenic detection of thiol-containing biomolecules using carbonaceous nanospheres loaded with silver nanoparticles as carrier.

Author information

1
Division of Nanomaterials & Chemistry, Hefei National Laboratory for Physical Sciences at Microscale, School of Chemistry and Materials, University of Science and Technology of China, Hefei 230026, People's Republic of China.

Abstract

Thiol-containing biomolecules show strong affinity with noble metal nanostructures and could not only stably protect them but also control the self-assembly process of these special nanostructures. A highly selective and sensitive chromogenic detection method has been designed for the low and high molecular weight thiol-containing biomolecules, including cysteine, glutathione, dithiothreitol, and bovine serum albumin, using a new type of carbonaceous nanospheres loaded with silver nanoparticles (Ag NPs) as carrier. This strategy relies upon the place-exchange process between the reporter dyes on the surface of Ag NPs and the thiol groups of thiol-containing biomolecules. The concentration of biomolecules can be determined by monitoring with the fluorescence intensity of reporter dyes dispersed in solution. This new chromogenic assay method could selectively detect these biomolecules in the presence of various other amino acids and monosaccharides and even sensitively detect the thiol-containing biomolecules with different molecular weight, even including proteins.

PMID:
21391719
DOI:
10.1021/nn2003053

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