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PLoS One. 2011 Feb 24;6(2):e17237. doi: 10.1371/journal.pone.0017237.

Specific oncogenic activity of the Src-family tyrosine kinase c-Yes in colon carcinoma cells.

Author information

1
Institut de Recherches Servier, Croissy-sur-Seine, France.

Abstract

c-Yes, a member of the Src tyrosine kinase family, is found highly activated in colon carcinoma but its importance relative to c-Src has remained unclear. Here we show that, in HT29 colon carcinoma cells, silencing of c-Yes, but not of c-Src, selectively leads to an increase of cell clustering associated with a localisation of β-catenin at cell membranes and a reduction of expression of β-catenin target genes. c-Yes silencing induced an increase in apoptosis, inhibition of growth in soft-agar and in mouse xenografts, inhibition of cell migration and loss of the capacity to generate liver metastases in mice. Re-introduction of c-Yes, but not c -Src, restores transforming properties of c-Yes depleted cells. Moreover, we found that c-Yes kinase activity is required for its role in β-catenin localisation and growth in soft agar, whereas kinase activity is dispensable for its role in cell migration. We conclude that c-Yes regulates specific oncogenic signalling pathways important for colon cancer progression that is not shared with c-Src.

PMID:
21390316
PMCID:
PMC3044743
DOI:
10.1371/journal.pone.0017237
[Indexed for MEDLINE]
Free PMC Article

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