Transplantation of a mammary stromal cell line into a mammary fat pad: development of the site-specific in vivo analysis system for mammary stromal cells

Biosci Biotechnol Biochem. 2011;75(3):550-5. doi: 10.1271/bbb.100773. Epub 2011 Mar 7.

Abstract

The interaction between mammary epithelial and stromal tissue is considered to be important in breast tissue development. In this study, we developed a transplantation procedure for the mammary stromal fibroblastic cell line (MSF) to examine its life in vivo. First we established MSF cells which stably expressed lacZ (lacZ/MSF) and had characteristics of mammary stromal cells. The lacZ/MSF cells were then transplanted into a cleared mammary fat pad of syngenic mice with and without mammary primary epithelial organoids. Whole mount X-gal and carmine staining of the transplants revealed that a number of undifferentiated lacZ/MSF cells survived around the mammary epithelial tissue when transplanted with organoids. These results indicate that transplantation of MSF cells into mammary fat pad was accomplished by co-transplantation with primary mammary organoids. Finally, we discuss the application of transplantation procedure for in vivo studies of the mammary stromal tissue development and stromal-epithelial interactions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue / cytology
  • Adipose Tissue / growth & development
  • Adipose Tissue / metabolism
  • Animals
  • Azo Compounds / analysis
  • Carmine / analysis
  • Cell Line
  • Epithelial Cells / cytology
  • Epithelial Cells / metabolism
  • Epithelium / growth & development
  • Epithelium / metabolism
  • Female
  • Fibroblasts / chemistry
  • Fibroblasts / cytology
  • Fibroblasts / metabolism
  • Fibroblasts / transplantation*
  • Galactosides / genetics
  • Galactosides / metabolism
  • Gene Expression
  • Immunohistochemistry
  • Indoles / metabolism
  • Keratins / analysis
  • Mammary Glands, Animal / cytology
  • Mammary Glands, Animal / growth & development
  • Mammary Glands, Animal / transplantation*
  • Mice
  • Organoids / cytology
  • Organoids / transplantation*
  • Paracrine Communication*
  • Stromal Cells / chemistry
  • Stromal Cells / cytology
  • Stromal Cells / metabolism
  • Stromal Cells / transplantation*
  • Transformation, Genetic
  • Transplantation, Isogeneic

Substances

  • Azo Compounds
  • Galactosides
  • Indoles
  • Keratins
  • Carmine
  • oil red O
  • 5-bromo-4-chloro-3-indolyl beta-galactoside