Abstract
The interaction between mammary epithelial and stromal tissue is considered to be important in breast tissue development. In this study, we developed a transplantation procedure for the mammary stromal fibroblastic cell line (MSF) to examine its life in vivo. First we established MSF cells which stably expressed lacZ (lacZ/MSF) and had characteristics of mammary stromal cells. The lacZ/MSF cells were then transplanted into a cleared mammary fat pad of syngenic mice with and without mammary primary epithelial organoids. Whole mount X-gal and carmine staining of the transplants revealed that a number of undifferentiated lacZ/MSF cells survived around the mammary epithelial tissue when transplanted with organoids. These results indicate that transplantation of MSF cells into mammary fat pad was accomplished by co-transplantation with primary mammary organoids. Finally, we discuss the application of transplantation procedure for in vivo studies of the mammary stromal tissue development and stromal-epithelial interactions.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adipose Tissue / cytology
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Adipose Tissue / growth & development
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Adipose Tissue / metabolism
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Animals
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Azo Compounds / analysis
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Carmine / analysis
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Cell Line
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Epithelial Cells / cytology
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Epithelial Cells / metabolism
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Epithelium / growth & development
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Epithelium / metabolism
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Female
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Fibroblasts / chemistry
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Fibroblasts / cytology
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Fibroblasts / metabolism
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Fibroblasts / transplantation*
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Galactosides / genetics
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Galactosides / metabolism
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Gene Expression
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Immunohistochemistry
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Indoles / metabolism
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Keratins / analysis
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Mammary Glands, Animal / cytology
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Mammary Glands, Animal / growth & development
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Mammary Glands, Animal / transplantation*
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Mice
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Organoids / cytology
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Organoids / transplantation*
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Paracrine Communication*
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Stromal Cells / chemistry
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Stromal Cells / cytology
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Stromal Cells / metabolism
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Stromal Cells / transplantation*
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Transformation, Genetic
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Transplantation, Isogeneic
Substances
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Azo Compounds
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Galactosides
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Indoles
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Keratins
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Carmine
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oil red O
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5-bromo-4-chloro-3-indolyl beta-galactoside