Format

Send to

Choose Destination
See comment in PubMed Commons below
J Cereb Blood Flow Metab. 2011 Jul;31(7):1623-36. doi: 10.1038/jcbfm.2011.17. Epub 2011 Mar 9.

Vascular phenotyping of brain tumors using magnetic resonance microscopy (μMRI).

Author information

1
The Whitaker Biomedical Engineering Institute at Johns Hopkins University School of Medicine, Baltimore 21205, Maryland, USA.

Erratum in

  • J Cereb Blood Flow Metab. 2011 Aug;31(8):1817-8.

Abstract

Abnormal vascular phenotypes have been implicated in neuropathologies ranging from Alzheimer's disease to brain tumors. The development of transgenic mouse models of such diseases has created a crucial need for characterizing the murine neurovasculature. Although histologic techniques are excellent for imaging the microvasculature at submicron resolutions, they offer only limited coverage. It is also challenging to reconstruct the three-dimensional (3D) vasculature and other structures, such as white matter tracts, after tissue sectioning. Here, we describe a novel method for 3D whole-brain mapping of the murine vasculature using magnetic resonance microscopy (μMRI), and its application to a preclinical brain tumor model. The 3D vascular architecture was characterized by six morphologic parameters: vessel length, vessel radius, microvessel density, length per unit volume, fractional blood volume, and tortuosity. Region-of-interest analysis showed significant differences in the vascular phenotype between the tumor and the contralateral brain, as well as between postinoculation day 12 and day 17 tumors. These results unequivocally show the feasibility of using μMRI to characterize the vascular phenotype of brain tumors. Finally, we show that combining these vascular data with coregistered images acquired with diffusion-weighted MRI provides a new tool for investigating the relationship between angiogenesis and concomitant changes in the brain tumor microenvironment.

PMID:
21386855
PMCID:
PMC3137465
DOI:
10.1038/jcbfm.2011.17
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Atypon Icon for PubMed Central
    Loading ...
    Support Center