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FEBS Lett. 2011 Apr 6;585(7):1049-53. doi: 10.1016/j.febslet.2011.03.002. Epub 2011 Mar 4.

The solution structure of coronaviral stem-loop 2 (SL2) reveals a canonical CUYG tetraloop fold.

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1
Department of Chemistry, Indiana University, Bloomington, IN 47405, USA.

Abstract

The transcription and replication of the severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) is regulated by specific viral genome sequences within 5'- and 3'-untranslated regions (5'-UTR and 3'-UTR). Here we report the solution structure of 5'-UTR derived stem-loop 2 (SL2) of SARS-CoV determined by NMR spectroscopy. The highly conserved pentaloop of SL2 is stacked on 5-bp stem and adopts a canonical CUYG tetraloop fold with the 3' nucleotide (U51) flipped out of the stack. The significance of this structure in the context of a previous mutagenesis analysis of SL2 function in replication of the related group 2 coronavirus, mouse hepatitis virus, is discussed.

PMID:
21382373
PMCID:
PMC3086565
DOI:
10.1016/j.febslet.2011.03.002
[Indexed for MEDLINE]
Free PMC Article
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